at the CNIC

Tag: brown adipose tissue (Page 1 of 6)

Predoctoral contract in metabolism

We are seeking a highly motivated candidate, who would like to start a scientific career doing their doctoral thesis working in understanding p38 kinases role in how the communiation between organs affect the metabolic. This work will be supervised by Dr. Guadalupe Sabio.

We offer incorporation to CNIC via an FPU predoctoral contract. Interested candidates will need to apply before 19 September 2023 in the following CNIC website:

METABOkines: Comunicación celular en el metabolismo: Implicación de la vía de las p38s.

PID2022-138525OB-I00

ELEGIBILITY CRITERIA

  • This call is open to applicants from all nationalities  holding a Master’s degree in Biomedical Sciences.
  • An excellent academic record and previous research experience during their undergraduate period will be valued very positively.
  • Authorship of publicacions in indexed journal will be valued very positively.
  • Candidates must have a solid working knowledge of English.

La Asociación Europea del Estudio de la Obesidad y la Fundación Novo Nordisk premian a Cintia Folgueira

Nuestra compañera Cintia Folgueira Cobos ha sido galardonada con el premio de la Asociación Europea del Estudio de la Obesidad y la Fundación Novo Nordisk en la categoría de Ciencias Básicas por su excelencia y compromiso.

El premio implica la concesión de una subvención de unos 40.000 euros y la participación de la Dra. Folgueira en el Congreso Europeo de la Obesidad, el 18 de este mes en Dublín. “Gracias a este reconocimiento conseguiremos identificar de manera más precisa los mecanismos moleculares que controlan la funcionalidad del tejido adiposo y la secreción de biomoléculas por el tejido adiposo, lo que podría resultar en el desarrollo de nuevos fármacos para combatir la obesidad y sus comorbilidades”, asegura.

Entre otros proyectos, la Dra. Folgueira está involucrada en el metabolismo del tejido adiposo y su función endocrina y termogénica.  “Mientras que el tejido adiposo blanco es un sitio de almacenamiento de energía, el tejido adiposo marrón disipa energía y es responsable de la termogénesis. Es en este tejido adiposo marrón y en este proceso de generación de calor (termogénesis) donde las mitocondrias son un componente central del consumo de energía y necesitan adaptarse a los cambios en las demandas energéticas: la activación mitocondrial es clave para regular la termogénesis”.

La importancia de las mitocondrias de la grasa

Sus investigaciones han encontrado que una proteína mitocondrial específica está disminuida en el tejido adiposo de pacientes obesos en comparación con individuos delgados. “En estudios con roedores, encontramos una disminución en el peso corporal y un aumento de la termogénesis cuando nuestros ratones son alimentados con dieta alta en grasa. Además, observamos que esta proteína está alterada después de la exposición al frío, lo que nos sugiere que puede controlar la termogénesis en el tejido adiposo marrón, lo que abre una ventana terapéutica para la prevención de la obesidad al modular su expresión”.

Modulation of hypothalamic AMPK phosphorylation by olanzapine controls energy balance and body weight

Vítor Ferreira, Cintia Folgueira, Maria Guillén, Pablo Zubiaur, Marcos Navares, Assel Sarsenbayeva, Pilar López Larrubia, Jan W. Eriksson, Maria J. Pereira, Francisco Abad-Santos, Guadalupe Sabio, Patricia Rada & Ángela M. Valverde.

Second-generation antipsychotics (SGAs) are a mainstay therapy for schizophrenia. SGA-treated patients present higher risk for weight gain, dyslipidemia and hyperglycemia. Herein, we evaluated the effects of olanzapine (OLA), widely prescribed SGA, in mice focusing on changes in body weight and energy balance. We further explored OLA effects in protein tyrosine phosphatase-1B deficient (PTP1B-KO) mice, a preclinical model of leptin hypersensitivity protected against obesity.

Olanzapine injection effects on AMPK phosphorylation (Image: Cintia Folgueira).

Wild-type (WT) and PTP1B-KO mice were fed an OLA-supplemented diet (5 mg/kg/day, 7 months) or treated with OLA via intraperitoneal (i.p.) injection or by oral gavage (10 mg/kg/day, 8 weeks). Readouts of the crosstalk between hypothalamus and brown or subcutaneous white adipose tissue (BAT and iWAT, respectively) were assessed. The effects of intrahypothalamic administration of OLA with adenoviruses expressing constitutive active AMPKα1 in mice were also analyzed.

Both WT and PTP1B-KO mice receiving OLA-supplemented diet presented hyperphagia, but weight gain was enhanced only in WT mice. Unexpectedly, all mice receiving OLA via i.p. lost weight without changes in food intake, but with increased energy expenditure (EE). In these mice, reduced hypothalamic AMPK phosphorylation concurred with elevations in UCP-1 and temperature in BAT. These effects were also found by intrahypothalamic OLA injection and were abolished by constitutive activation of AMPK in the hypothalamus. Additionally, OLA i.p. treatment was associated with enhanced Tyrosine Hydroxylase (TH)-positive innervation and less sympathetic neuron-associated macrophages in iWAT. Both central and i.p. OLA injections increased UCP-1 and TH in iWAT, an effect also prevented by hypothalamic AMPK activation. By contrast, in mice fed an OLA-supplemented diet, BAT thermogenesis was only enhanced in those lacking PTP1B. Our results shed light for the first time that a threshold of OLA levels reaching the hypothalamus is required to activate the hypothalamus BAT/iWAT axis and, therefore, avoid weight gain.

Our results have unraveled an unexpected metabolic rewiring controlled by hypothalamic AMPK that avoids weight gain in male mice treated i.p. with OLA by activating BAT thermogenesis and iWAT browning and a potential benefit of PTP1B inhibition against OLA-induced weight gain upon oral treatment.

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